Qualitative Identification and Content Determination of Berberine Hydrochloride in Huangjin Paste / 中国药房
China Pharmacy
;
(12): 4262-4263,4264, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-605219
ABSTRACT
OBJECTIVE:
To establish a method for the qualitative identification and content determination of berberine hydro-chloride in Huangjin paste.METHODS:
TLC was adopted for the qualitative identification of berberine hydrochloride and HPLC was conducted for the content determination of berberine hydrochloride in preparation. The column was Symmetry Shield Rp-18 with mobile phase of acetonitrile-0.1% phosphoric acid(50∶50,V/V)at a flow rate of 1.0 ml/min,detection wavelength was 265 nm,column temperature was 30 ℃ and injection volume was 10 μl.RESULTS:
TLC spots of berberine hydrochloride in prepara-tion were clear and well-separated. The linear range of berberine hydrochloride was 2.5-20.0 μg/ml(r=0.999 0);RSDs of preci-sion,stability and reproducibility tests were lower than 3%;recovery was 97.7%-102.1%(RSD=1.68%,n=6). CONCLUSI-ONSThe method is simple,accurate and reproducible,and can be used for the qualitative identification and content determination of berberine hydrochloride in Huangjin paste.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio diagnóstico
/
Investigación cualitativa
Idioma:
Chino
Revista:
China Pharmacy
Año:
2015
Tipo del documento:
Artículo
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