Research on 3D printed β-TCP/HA/PLA scaffolds for bone grafts / 国际生物医学工程杂志

ABSTRACT

Objective To develop the personalized 3D printing scaffolds for bone grafts,to meet the needs of the patients with the bone defect.Methods The model of three-dimensional woodpile structure was designed by the software of computer-aided design (CAD).The β-tricalcium phosphate (β-TCP),hydroxyapatite (HA),and polylactic acid (PLA) composite scaffolds with three-dimensional woodpile structure was simulated using 3D printing method by three-dimensional air floating platform.The three-dimensional bone graft scaffolds was then vacuum heat-treated,and the residual chloroform was detected by energy dispersive X-ray spectrometer.The morphology of the β-TCP/HMPLA scaffolds was characterized by scanning electron microscope,and in vitro cytotoxicity against osteoblasts hFOB 1.19 was assessed by thiazolyl blue tetrazolium bromide (MTT) assay.Results When the extrusion pressure of printing slurry was 137.9-413.7 kPa,the three-dimensional bone graft scaffolds could be printed out.Then the scaffolds was vacuum treated at 90 ℃ and preheated at 150 ℃,which could eliminate the solvent CHC13.The three-dimensional bone graft scaffolds,with a through-hole and rough surface,was co-cultured with osteoblasts hFOB1.19 for 7 d,and it's cytotoxicity grade was grade 0.Conclusions The three-dimensional bone graft scaffolds have a through-hole and rough surface,which is favorable to the osteoblasts culture and bone induction,indicating the advantage and development prospects of 3D printing in the preparation of porous materials for bone grafts.