Effects of Aerobic Exercise on the Expression of Hepatic Hepcidin, and the Level of Serum IL-6 and MCP-1 in ApoE-/-Mice / 中国运动医学杂志

ABSTRACT

Objective To observe the preventive effects of the aerobic exercise on the expression of hepatic hepcidin,and the level of serum IL-6 and MCP-1 in ApoE-/-rats with atherosclerosis (AS) and to explore the mechanism.Methods Twenty 8-week male ApoE-/-mice were randomly divided into a control group and an exercise group,each of 10.The AS model was established in both groups through feeding high-fat diet.The control group was not given exercise intervention,while the exercise group underwent 14-week progressive intensity treadmill training,5 times a week,starting at 10 n/min for 30 min for the first 5 weeks and increasing to 13 m/min for 60 min at the end.At the end of the experiment,aortic samples were fixed and treated using the hematoxylin-eosin staining and Oil Red O.Then the pathological changes of the aorta were analyzed.The hepcidin expression of liver and serum hepcidin was assessed using Western blotting,and the level of the,interleukelin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1) were detected using ABC-ELISA.Results The pathological slice showed that the vascular fiber plate and internal membrane of the aorta in the exercise group were better than that of the control group,with less ruptured vascular fibreboards,foam cell infiltration and fiber plaques.The hepcidin expression of liver and the level of serum hepcidin,the level of the serum IL-6 and MCP-1 in the exercise group were significantly lower than those of the control group (P＜0.01 and P＜0.01).Conclusion The long-term aerobic exercise can prevent AS for ApoE /-mice through lowering the expression of the hepatic hepcidin and serum hepcidin,as well as the level of serum IL-6 and MCP-1.