Overexpression of IL-18 gene by T lymphocytesenhances their cytotoxic effect against pancreatic cancer cells SW-1990 / 基础医学与临床
Basic & Clinical Medicine
;
(12): 1251-1256, 2017.
Artículo
en Chino
| WPRIM
| ID: wpr-609279
ABSTRACT
Objective To investigate the antitumor effects of IL-18 gene transfected T cells on pancreatic cancer cell SW-1990.Methods Construction of IL-18 contained recombinant lentivirus using PCR method and packaged at HEK293T cell, then transfected the human T cells and evaluated the antitumor effects when cocultured with SW-1990.Results The IL-18 gene contained recombinant lentivirus was successfully constructed and packaged, and transfected the T cells, the LDH secretion and IL-2 and IFN-γ content all increased significantly (P<0.01) when cocultured with SW-1990 cell.Conclusions T cells transfected with IL-18 possessed more potent antitumor effects to pancreatic cancer cell SW-1990 as compared to the regular T cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Basic & Clinical Medicine
Año:
2017
Tipo del documento:
Artículo
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