Expression and significance of microRNA-195 and Bcl-2 mRNA in the childhood acute lymphoblastic leukemia cells / 中华实用儿科临床杂志

ABSTRACT

Objective To investigate the expression and significance of microRNA-195 (miR-195) and Bcl-2 in different type of childhood acute lymphoblastic leukemia (ALL) cells.Methods A total of 110 patients diagnosed as ALL from the Pediatrics Department and Outpatient Department of the First Affiliated Hospital of Zhengzhou University from July 2010 to December 2016 were recruited.The patients included newly diagnosed group,remission group and relapse group in which the newly diagnosed group was divided into standard risk(SR) group,intermediate risk (IR) group and high risk (HR) group.And another 14 gender-and age-matched children received bone orthopaedic surgery who excluded tumor and blood system diseases in the same hospital were recruited as control group during the same period.Bone marrow samples of 2-5 mL were drawn from all the subjects.The samples of miR-195 and the expression of Bcl-2 mRNA were detected by using quantitative real time polymerase chain reaction,while the expression of Bcl-2 protein was also detected by using Western blot.Results MiR-195 expression was lower in the newly diagnosis and relapse ALL children (SR1.25 ± 0.15;IR1.63 ± 0.54;HR0.78 ± 0.35;relapse0.46 ± 0.18),compared with the control group,and there were statistically significant differences (t =42.44,28.63,41.35,42.65,all P =0.000),but the expression of Bcl-2 mRNA was higher in the newly diagnosis and relapse ALL children than that in the control group (SR2.56 ± 0.91;IR4.52 ± 0.43;HR5.49 ± 1.28;relapse5.32 ± 1.15),which had statistically significant differences (t =3.58,9.86,10.12,8.81,all P =0.000).With the rising of the criticality of ALL,the expression of miR-195 reduced gradually,and the expression of Bcl-2 mRNA increased step by step.Determining the protein of Bcl-2 detected by Western blot showed the same result.Conclusions The lower expression of miR-195 and the higher expression of Bc1-2 may be a marker to distinguish the risk factors of ALL,which may be important for the diagnosis and the determination of the degree of risk for the ALL children.