Effects of Apigenin on Renal Ischemia/reperfusion Injury Apoptosis in Rats by Fas/FasL / 医学研究杂志

ABSTRACT

Objective To investigate the effect of apigenin and the change of expression of Fas/FasL after renal ischemia/reperfusion injury in rats.Methods Establishing Renal ischemia/reperfusion injury rat model was established.Sixty rats were randomly divided into 5 Groups.Blood samples were collected for the detection of serum creatinine and urea nitrogen levels.The level of SOD,MDA and GSH-Px were detected.Histologic examinations were evaluated.The expression of Fas,FasL,Caspase-3 and Bcl-2 were performed by immunohistochemistry and Western blot.Results Compared with the sham group,the Cr and BUN levels in the I/R groups of were significantly increased,the level of SOD and GSH-Px reduced,the level of MDA improved.Histopathological damage of renal tubular aggravated.The immunohistochemical expression of FasL and Caspase-3 as shown in enhanced and Bcl-2 reduced.Western blot showed that Fas and FasL expression increased and Bcl-2 reduced significantly,and the difference was statistically significant (P ＜ 0.05).Compared with I/R group,apigenin significantly enhanced renal function,and alleviated renal tubular epithelial cell injury and decreased Fas,FasL and Caspase-3 expression,improved Bcl-2 expression,difference were statistically significant (P ＜ 0.05).Conclusion Apigenin can protect the rats subjected to ischemia/reperfusion injury.Its protective mechanism may be related to the inhibition in expression of Fas/FasL and the increase in expression of Bcl-2.