Mast cells promote the beiging of white adipose tissue from cold shock / 中华内分泌代谢杂志

ABSTRACT

This article is the translation of Mast Cells Promote Seasonal White Adipose Beiging in Humans, published in the Diabetes in May, 2017(Diabetes 2017,661237-1246), with the consent of Diabetes.This study mainly investigated the mechanism of the beiging of white adipose tissue(WAT).Studies in rodents suggested that cold shock and a number of immune mediators were important for the beiging of subcutaneous white adipose tissue (SC-WAT).This study observed the seasonal beiging of SC-WAT from lean humans, measured the gene expression of various immune cell markers, and performed multivariate analysis of the gene expression data to identify genes that could predict uncoupling protein-1(UCP-1) expression.The mechanism of the induction of UCP-1 from adipocytes by mast cells was also explored by in vitro experiment.The results showed that TIB64 mast cells responded to cold shock by releasing histamine and interleukin 4 (IL-4) which promoted the expression of UCP-1 and the lipolysis of 3T3-L1 adipocytes.Pharmacological block of the degranulation of mast cells potently inhibited the release of histamine and adipocyte UCP-1 mRNA induction by conditioned medium.In summary, mast cells are an important immune cell type in the beiging of WAT which sense colder temperatures, and release factors that promote UCP-1 expression.