The experimental research of Neutrophil Gelatinase-associated Lipocalin(NGAL) siRNA encapsulated by UAC on NGAL gene silence in colon cancer cells / 中国生化药物杂志
Chinese Journal of Biochemical Pharmaceutics
;
(6): 14-17, 2017.
Artículo
en Chino
| WPRIM
| ID: wpr-613955
ABSTRACT
Objective To explore the effect of NGAL knockdown by NGAL siRNA encapsulated with urocanic acid-modified chitosan nanoparticles (UAC).MethodsNGAL siRNA encapsulated by UAC and chitosan (CTS) respectively, which were then used to transfect human colon cancer cell lines HT29.The expression level of NGAL protein were detected by Enzyme Linked Immunosorbent Assay(ELISA).ResultsThe ELISA study revealed that the expression level of NGAL protein in UAC group(average 0.583μg/L) was significantly lower than in CTS group (average 0.772μg/L) and control group(average 1.071μg/L) (P<0.05).ConclusionThe NGAL expression of mRNA and protein in HT29 cells could be down-regulated by siRNA encapsulated by UAC.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Biochemical Pharmaceutics
Año:
2017
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS