Model of neuroinflammation associated with cognitive dysfunction established by intraperitoneal injection of LPS / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12): 890-895, 2017.
Artículo
en Chino
| WPRIM
| ID: wpr-613997
ABSTRACT
AIM:
To establish a mouse model of immuno-inflammation in central nervous system (CNS) associated with cognitive dysfunction.METHODS:
C57BL/6J male mice were divided into 3 groups.Lipopolysaccharide (LPS) was intraperitoneally injected into the mice to induce cognitive impairment.Morris water maze test, passive avoidance test and pole test were used to observe the behavioral changes of mice.The histomorphology was analyzed by the method of immunofluorescence.The detailed molecular mechanism was determined by Western blot.RESULTS:
Compared with saline group, LPS induced mouse sickness behavior and memory loss.Microglia activation and neuronal loss in the hippocampus were observed.The expression of neuroinflammatory proteins COX-2 and iNOS in the brain of LPS-induced mice was increased.CONCLUSION:
Intraperitoneal injection of LPS induces cognitive dysfunction in mice.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Pathophysiology
Año:
2017
Tipo del documento:
Artículo
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