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Impact of Wildtype p53 Induced Phosphatase 1 Gene Lacking on Heart Function in Experimental Mice / 中国循环杂志
Chinese Circulation Journal ; (12): 792-796, 2017.
Artículo en Chino | WPRIM | ID: wpr-614142
ABSTRACT

Objective:

To explore the impact of knocking out wildtype p53 phosphatase 1 gene on heart function with the changes of cardiac tissue mRNA and protein expressions in experimental mice.

Methods:

Our research included in 2 groups Wildtype (WT) mice group and Wip1 knockout (Wip1-KO) mice group. n=10 in each group. The heart function, ratio of heart weight to body weight (HW/BW) were examined and compared between 2 groups; cardiac tissue morphology was observed by HE staining; mRNA expressions of ANP, BNP, MCP-1 andα-SMA were determined by RT-PCR and protein expressions of Bcl-2, Bax and c-caspase3 were measured by Western blot analysis.

Results:

Compared with WT mice group, Wip1-KO mice group showed decreased Wip1 mRNA expression,P0.05; apoptosis related protein expressions of Bax/Bcl-2 and c-caspase3 were similar between 2 groups,P>0.05.

Conclusion:

Wip1 gene knockout may impair the heart function in experimental mice, while the relevant mechanism should be further investigated.

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Chinese Circulation Journal Año: 2017 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Chinese Circulation Journal Año: 2017 Tipo del documento: Artículo