Your browser doesn't support javascript.
loading
Comparison of viability of oral epithelial cells stored by different freezing methods / 대한치과보존학회지
Journal of Korean Academy of Conservative Dentistry ; : 491-499, 2009.
Artículo en Inglés | WPRIM | ID: wpr-61599
ABSTRACT
This study examined the influence of the storage methods on the viability of oral epithelial cells using conventional cell freezing storage, slow freezing preservation, rapid freezing preservation, and slow freezing preservation with a pressure of 2 Mpa or 3 Mpa. The cell viability was evaluated by cell counting, WST-1 and the clonogenic capacity after 6 days of freezing storage. After 6 days, the frozen cells were thawed rapidly, and the cell counting, WST-1, and clonogenic capacity values were measured and compared. 1. The results from cell counting demonstrated that conventional cryopreservation, slow freezing under a 2 Mpa pressure and slow freezing under a 3 Mpa pressure showed significantly higher values than slow freezing preservation and rapid freezing preservation (p<0.05). 2. The results from the optical density by WST-1 demonstrated that slow freezing under a 2 Mpa pressure showed significantly higher values than slow freezing preservation and rapid freezing preservation (p<0.05). 3. The clonogenic capacity demonstrated that slow freezing under a 2 Mpa pressure showed significantly higher values than slow freezing preservation and rapid freezing preservation (p<0.05).
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Criopreservación / Recuento de Células / Supervivencia Celular / Células Epiteliales / Congelación Idioma: Inglés Revista: Journal of Korean Academy of Conservative Dentistry Año: 2009 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Criopreservación / Recuento de Células / Supervivencia Celular / Células Epiteliales / Congelación Idioma: Inglés Revista: Journal of Korean Academy of Conservative Dentistry Año: 2009 Tipo del documento: Artículo