Clinical value of peripheral blood Runx3 gene CpG island's methylation in the evaluation of colon cancer's disease condition and prognosis / 中国现代普通外科进展

ABSTRACT

Objective:To study the clinical value of peripheral blood Runx3 gene CpG island's methylation in the evaluation of colon cancer's disease condition and prognosis.Methods:Methy1ation specific PCR was used to detect the Runx3 gene CpG island's methylation by Peripheral blood in colon cancer patient (observation group) and healthy people (control group).The Runx3 gene CpG island's methylation rates in different clinicopathological factors were compared.The 3 year survival rate and total survival time between patient with Runx3 gene CpG island's methylation and unmethylation were Compared.Result:The Runx3 gene CpG island's methylation rate in observation group was significantly higher than in control group (P＜0.05).The Runx3 gene CpG island's methylation rates in degree of differentiation,maximum tumor diameter,lymph node metastasis,distant metastasis,TNM staging and surgical resection were significant different (P＜0.05).The 3 year survival rate and total survival time in patient with Runx3 gene CpG island's methylation were significantly higher than in patient with Runx3 gene CpG island's unmethylation.Conclusion:Runx3 gene CpG island is hypermethylated in colon cancer's peripheral blood,which has value in the evaluation of colon cancer's disease condition and prognosis.