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Dilution validation of high level D-dimer samples in Sysmex CS5100 instrument / 国际检验医学杂志
International Journal of Laboratory Medicine ; (12): 1630-1633, 2017.
Artículo en Chino | WPRIM | ID: wpr-619450
ABSTRACT
Objective To investigate the necessity of dilution in samples with high concentrations of D-Dimer and optimum dilution multiple.Methods Quality control products and calibration were detected by using Sysmex CS5100 for precision evaluation,including within-batch and between-run precision.Calibration were detected for validation of linear range and clinical reportable.Samples with D-Dimer5 mg/L and FDP>20 μg/mL were also serially diluted and detected to calculated recovery rate.Results Within-batch and between-run coefficients of variation were both less than 3%.Within the scope of 0.207-5.170 mg/L,the linear distribution was fine.The clinical reportable range was 0.207-165.440 mg/L.For samples with D-Dimer5 mg/L and FDP>20 μg/mL,there was obvious antigen excess phenomenon,and gradient dilution was required.Conclusion For samples with D-Dimer>5 mg/L and FDP>20 μg/mL,dilution should be performed to ensure the accuracy of detected results.

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: International Journal of Laboratory Medicine Año: 2017 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: International Journal of Laboratory Medicine Año: 2017 Tipo del documento: Artículo