Effect of etomidate on activity of nuclear factor kappa B during lung ischemia-reperfusion injury in rats / 中华麻醉学杂志

ABSTRACT

Objective To evaluate the effect of etomidate on the activity of nuclear factn kappa B (NF-κB) during lung ischemia-reperfusion (I/R) injury in rats.Methods Sixty SPF healthy adult male Sprague-Dawley rats,weighing 180-220 g,were divided into 4 groups (n =15 each) using a random number table:sham operation group (group S),lung I/R group (group I/R),etomnidate group (group E) and fat emulsion group (group F).Lung I/R was induced by 45 min occlusion of the left hilum of the lung followed by 120 min reperfusion.At 3 min before reperfusion,normal saline 1 ml was injccted via the auricular vein in I/R and S groups,and etomidate 0.3 mg/kg and fat emulsion 0.3 mg/kg (diluted to l nl in normal saline) were injected via the auricular vein in E and F groups,respectively.The rats were sac rificed at the end of reperfusion,and the lungs were removed for examination of the pathological changes (using haematoxylin and eosin staining) and for determination of the wet to dry weight ratio (W/D ratio),cell apoptosis (by TUNEL),expression of NF-κB in nucleoprotein (by Western blot) and content of tumor necrosis factor-alpha (TNF-α) in lung tissues (by enzyme-linked immunosorbent assay).Apoptotic index (AI) was calculated.Results Compared with group S,the W/D ratio,AI and content of TNF-α in lung tissues were significantly increased,and the expression of NF-κB in nucleoprotein was up-regulated in group I/R (P＜0.05).Compared with group I/R,the W/D ratio,AI and content of TNF-α in lung tissues were significantly decreased,and the expression of NF-κB in nucleoprotein was down-regulated in group E (P＜0.05),and no significant change was found in the parameters mentioned above in group F (P＞0.05).The pathological changes of the lung were significantly attenuated in group E as compared with group I/R.Conclusion The mechanism by which etomidate reduces lung I/R injury is related to inhibition of NF-κB activity in rats.