Study on the vaginal bacteria test by PCR and bacterial culture / 中国生化药物杂志

ABSTRACT

Objective To study the effect of PCR and bacterial culture in vaginal bacteria test.Methods A total of 84 patients were enrolled in the department of obstetrics and gynecology, affiliated hospital of Nankai university, from February 2014 to March 2016.Vaginal secretion was collected and tested by PCR(group A, n=42), and bacterial culture(group B, n=42).Vaginal bacteria test Resultswere compared in the two groups.ResultsEnterococcus were positive in 13 cases(30.95%)in group A, 3 cases(7.14%)in group B, gardnerella vaginalis were positive in 10 cases(23.81%)in group A, 19 cases(45.24%)in group B;Corynebacterium were positive in 13 cases(30.95%)in group A, 5 cases(11.91%)in group B.Negative rate of vaginal bacterial test was 14.29% in group A, 35.71% in group B.In the above data, the differences between the 2 groups were statistically significant(P<0.05).Conclusion PCR test can effectively improve the positive rate of vaginal bacterial examination, and provide an objective basis for clinical treatment.