The value of multiple immunological indexes in the identification of anaphylactic death by penicillin / 中国法医学杂志

ABSTRACT

Objective To investigate the expression level of tryptase, chymase, IL-4 and IL-10 in guinea pigs died from anaphylactic death caused by penicillin allergy within 48 hours. Methods Guinea pigs were sensitized and elicited by penicilloyl-protein, the blood and tissues were extracted within 48 hours after the death. The expression of tryptase and chymase in tracheas and lungs were detected by the ways of immunohistochemistry, IL-4 and IL-10 levels in serum, tracheas and lungs were detected by ELISA. Results Compared with the control group, the expression of tryptase and chymase has enhanced in lungs and tracheas, the level of IL-4 and IL-10 increased in serum, lungs and tracheas in experimental groups(P<0.05). Conclusion Tryptase, chymase, IL-4 and IL-10 have significant value in the identification of the deaths caused by penicillin allergy.