Effect of exogenous hydrogen sulfide on phenotypic transformation of alveolar macrophages in a mouse model of endotoxin-induced acute lung injury / 中华麻醉学杂志

ABSTRACT

To evaluate the effect of exogenous hydrogen sulfide on phenotypic transformation of alveolar macrophages in a mouse model of endotoxin-induced acute lung injury (ALI).Methods Thirty pathogen-free healthy male C57BL/6 mice,aged 8 weeks,weighing 18-20 g,were divided into 3 groups (n =10 each) using a random number table:sham operation group (group Sham),group ALI and exogenous hydrogen sulfide group (group NaHS).In group Sham,normal saline was intratracheally instilled and intraperitoneally injected.In ALI and NaHS groups,lipopolysaccharide 20 mng/kg was intratracheally instilled,and normal saline and sodium hydrosulfide (28 μmol/kg) 100 μl were intraperitoneally injected,respectively,every day.Mice were sacrificed at day 3 after administration of lipopolysaccharide,and lungs were removed for measuremnent of the lung coefficient and expression of inducible nitric oxide synthase (iNOS) and arginase (by immunohistochemistry) and for microscopic examination of the pathological changes.Lung injury was evaluated by the index of quantitative assessment (IQA).Results Compared with group Sham,the lung coefficient and IQA were significantly increased,and the expression of iNOS and arginase in lung tissues was up-regulated in group ALI (P＜0.05).Compared with group ALI,the lung coefficient and IQA were significantly decreased,the expression of iNOS in lung tissues was down-regulated (P＜0.05),and no significant change was found in the expression of arginase in lung tissues in group NaHs (P＞0.05).Conclusion Exogenous hydrogen sulfide mitigates endotoxin-induced ALI through inhibiting phenotypic transformation of alveolar macrophages to M1 subtype in mice.