THE IMPROVEMENT OF RT-PCR TECHNIQUE ON DETECTING ROTAVIRUS / 药物分析学报

ABSTRACT

Objective To establish a speed and effective method to detect rotavirus. Methods Using ELISA and one step RT-PCR to detect 196 clinic samples from Xi'an area. Results Compared with ELISA method, one step RT PCR was more sensitive and specific (P ＜0.05). Conclusion One step RT-PCR is a simple, speed, sensitive and spe cific method for clinic and epidemic studies of rotavirus.