Cloning and expression of the 1.2kb cDNA fragment of human integrin α 4 subunit / 细胞与分子免疫学杂志
Chinese Journal of Cellular and Molecular Immunology
; (12): 13-15, 2001.
Article
en Zh
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| ID: wpr-622188
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ABSTRACT
Aim To clone and express the 1.2kb cDNA fragment (1/753-2 934bp) of human integrin α 4 subunit. Methods The 1.2 kb cDNA fragment of human integrin α 4 subunit was amplified from HL-60 total RNA by RT-PCR, then it was subcloned into expression vector pGEX-3X and induced with IPTG. Results The 1.2 kb cDNA fragment of human integrin α 4 subunit was cloned. The sequencing indicated that there was only one missense mutation (Arg→ Gln) among the fragment, and this mutation won't affect antigenicity after analysed by GOLDKEY. Then the 1.2 kb cDNA was subcloned into expression vector pGEX-3X. The α 4 fragment was highexpressed in E.coli after induced with IPTG. Conclusion The 1.2kb cDNA fragment of α 4 subunit was obtained, and it was highexpressed in E.coli, it might be important for study on the function of α 4 integrins.
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WPRIM
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Zh
Revista:
Chinese Journal of Cellular and Molecular Immunology
Año:
2001
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Article