The apoptosis of mouse macrophage J774A. 1 induced by oxysterol depend on NF-κB activation / 细胞与分子免疫学杂志
Chinese Journal of Cellular and Molecular Immunology
;
(12): 879-882, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-622335
ABSTRACT
AIM:
Investigated the relationship between NF-κB activation and cell apoptosis in mouse macrophages treated with 7-ketochesterol (7-KC).METHODS:
Cell apoptosis was detected by MTT assay, DNA fragmentation assay and flow cytometric analysis. NF-κB activation was detected by western blot and immunohistochemistry. Inhibitory assay was used to show the effect of the activation of NF-κB on the apoptosis induced by 7-KC.RESULTS:
7-KC inhibited macrophages proliferation, and then induced apoptosis, which is associated with NF-κB activation. Moreover, cell apoptosis with NF-κB activation was inhibited by pyrrolidine dithiocarbamate (PDTC), an inhibitor of NF-κB.CONCLUSION:
7-KC induced the activation of NF-κB and following cell apoptosis.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Cellular and Molecular Immunology
Año:
2009
Tipo del documento:
Artículo
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