Detection of MAGE and SSX Gene Expressions by RT-nested PCR Using Common Primers in Head and Neck Cancer
Clinical and Experimental Otorhinolaryngology
;
: 97-102, 2008.
Artículo
en Inglés
| WPRIM
| ID: wpr-62422
ABSTRACT
OBJECTIVES:
The melanoma antigen gene (MAGE) and synovial sarcoma on X chromosome (SSX) gene families are silent in most normal adult tissues, but are expressed in a variety of malignant lesions. Therefore, detection of MAGE and SSX transcription may be useful for the diagnosis of head and neck cancers. The aim of this study is to detect MAGE and SSX gene transcripts of head and neck cancers using the MAGE 1-6 assay and the SSX 1-9 assay.METHODS:
The transcripts of MAGE 1-6 and SSX 1-9 genes were detected by the MAGE 1-6 assay and the SSX 1-9 assay respectively, in cancer cell lines, cancer tissue, and induced sputum specimens from head and neck cancer patients.RESULTS:
The transcripts of MAGE 1-6 and SSX 1-9 genes were detected in 82.8% and 75.9% of head and neck cancer tissues (N=29) respectively, and 96.6% of cancer tissues expressed at least one of MAGE 1-6 or SSX 1-9 genes. In the induced sputum of head and neck cancer patients (N=18), the transcripts of MAGE 1-6 and SSX 1-9 genes were detected in 72.2% and 77.8%, respectively, and 94.4% of the sputum specimens were positive for either the MAGE 1-6 or the SSX 1-9 assay.CONCLUSION:
These results suggest that the combination of MAGE 1-6 and SSX 1-9 assays may be useful in the diagnosis of head and neck cancer.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Esputo
/
Cromosoma X
/
Expresión Génica
/
Línea Celular
/
Reacción en Cadena de la Polimerasa
/
Sarcoma Sinovial
/
Cabeza
/
Neoplasias de Cabeza y Cuello
/
Melanoma
/
Cuello
Tipo de estudio:
Estudio diagnóstico
Límite:
Adulto
/
Humanos
Idioma:
Inglés
Revista:
Clinical and Experimental Otorhinolaryngology
Año:
2008
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS