Strategy for generating tissue-engineered human bone construct
The Medical Journal of Malaysia
;
: 43-44, 2004.
Artículo
en Malayalam
| WPRIM
| ID: wpr-629925
ABSTRACT
The strategy used to generate tissue-engineered bone construct, in view of future clinical application is presented here. Osteoprogenitor cells from periosteum of consenting scoliosis patients were isolated. Growth factors viz TGF-B2, bFGF and IGF-1 were used in concert to increase cell proliferation during in vitro cell expansion. Porous tricalcium phosphate (TCP)-hydroxyapatite (HA) scaffold was used as the scaffold to form 3D bone construct. We found that the addition of growth factors, greatly increased cell growth by 2 to 7 fold. TCP/HA proved to be the ideal scaffold for cell attachment and proliferation. Hence, this model will be further carried out on animal trial.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Técnicas de Cultivo de Órganos
/
Periostio
/
Regeneración Ósea
/
División Celular
/
Colágeno
/
Trasplante Óseo
/
Ingeniería de Tejidos
/
Trasplante de Células Madre Mesenquimatosas
Tipo de estudio:
Estudio pronóstico
Idioma:
Malayalam
Revista:
The Medical Journal of Malaysia
Año:
2004
Tipo del documento:
Artículo
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