The effects of age on monolayer culture of human keratinocytes for future use in skin engineering
The Medical Journal of Malaysia
;
: 182-183, 2004.
Artículo
en Malayalam
| WPRIM
| ID: wpr-629956
ABSTRACT
Skin is the largest organ in human system and plays a vital role as a barrier against environment and pathogens. Skin regeneration is important in tissue engineering especially in cases of chronic wounds. With the tissue engineering technology, these skins equivalent have been use clinically to repair burns and wounds. Consented redundant skin samples were obtained from patients aged 9 to 65 years old. Skin samples were digested with dispase, thus separating the epidermis and the dermis layer. The epidermis layer was trypsinized and cultured in DKSFM in 6-well plate at 37 degrees C and 5% CO2. Once confluent, the culture were trypsinized and the cells were pooled. Cells were counted using haemacytometer. Doubling time and viability were calculated and analysed. From the result, we conclude that doubling time and viability of in vitro keratinocytes cultured in DKSFM media is not age dependant.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Cicatrización de Heridas
/
Heridas y Lesiones
/
Quemaduras
/
Queratinocitos
/
División Celular
/
Supervivencia Celular
/
Enfermedad Crónica
/
Factores de Edad
/
Trasplante de Piel
/
Senescencia Celular
Idioma:
Malayalam
Revista:
The Medical Journal of Malaysia
Año:
2004
Tipo del documento:
Artículo
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