Phenotypic expression of collagen type II and collagen type I gene in monolayer culture of human auricular chondrocytes
The Medical Journal of Malaysia
;
: 188-189, 2004.
Artículo
en Malayalam
| WPRIM
| ID: wpr-629959
ABSTRACT
Cartilage is regularly needed for reconstructive surgery. Basic research in tissue engineering is necessary to develop its full potential. We presented here the expression profile of type II collagen gene and type I collagen gene in human auricular monolayer culture expansion. Cultured chondrocytes documented a reduction in the expression level of collagen type II gene whilst collagen type I gene was gradually expressed through all the passages. This study demonstrated that human auricular chondrocytes lose its phenotypic expression during monolayer culture expansion. Further studies are required to enhance cartilage specific gene expression, collagen type II throughout the in vitro culture.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fenotipo
/
Células Cultivadas
/
Condrocitos
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Colágeno Tipo I
/
Colágeno Tipo II
/
Ingeniería de Tejidos
/
Oído Externo
/
Fibroblastos
Idioma:
Malayalam
Revista:
The Medical Journal of Malaysia
Año:
2004
Tipo del documento:
Artículo
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