Whole CagA gene amplification of Helicobacter pylori and its fingerprinting by restriction fragment length polymorphism / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
;
(6): 276-8, 2002.
Artículo
en Inglés
| WPRIM
| ID: wpr-634097
ABSTRACT
To set up a method of amplification for the whole CagA gene of Helicobacter pylori and its fingerprinting by restriction fragment length polymorphism (RFLP), nested PCR was employed in combination with TD-PCR to amplify the gene and EcoRI and Hind III were used to generate the RFLP fingerprinting. Target DNA fragments from 13 of 20 samples were successfully amplified and the relevant RFLP fingerprintings were obtained. It is concluded that the method can be used to amplify the whole CagA gene and CagA gene has apparent diversity of RFLP profile.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas Bacterianas
/
Polimorfismo de Longitud del Fragmento de Restricción
/
Amplificación de Genes
/
Dermatoglifia del ADN
/
Helicobacter pylori
/
Antígenos Bacterianos
Idioma:
Inglés
Revista:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Año:
2002
Tipo del documento:
Artículo
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