Standard and quantitative analysis of cyclin E threshold by cyclin E/DNA multiparameter flow cytometry / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
;
(6): 282-4, 2005.
Artículo
en Inglés
| WPRIM
| ID: wpr-634268
ABSTRACT
The threshold of cyclin E expression at G1/S boundary is a characteristic feature of cell cycle progressing. In this study, we tried to develop a quantitative approach to analyze cyclin E threshold by multiparameter flow cytometry. The expression of cyclin E in exponentially growing MOLT-4 cells was detected under different photomultiplier tube (PMT) voltages by cyclin E/DNA multiparameter flow cytometry. Additionally, cyclin E was detected in cells which were treated with caffeine and cycloheximide (CHX) under the same PMT voltage. Moreover, the expression of cyclin E in MOLT-4 cells was compared with that in JURKAT cells. Cyclin E threshold was quantified by formula B2/AxC (A, B, C indicates the minimum, threshold, and maximum of cyclin E fluorescence intensity, respectively). Results showed that in MOLT-4 cells, cyclin E threshold calculated by formula B2/AxC was invariable under different PMT settings. It was decreased in cells treated with caffeine and remained changeless in cells treated with cycloheximide. Cyclin E threshold in JURKAT cells was much lower than that in MOLT-4 cells. It was suggested that Formula B2/AxC we firstly set up could be used to analyze cyclin E expression threshold quantitatively.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Cafeína
/
ADN de Neoplasias
/
Leucemia Linfoide
/
Ciclo Celular
/
Células Jurkat
/
Ciclina E
/
Cicloheximida
/
Línea Celular Tumoral
/
Citometría de Flujo
Idioma:
Inglés
Revista:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Año:
2005
Tipo del documento:
Artículo
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