Observation of the Expression of HCV NS 5 Antigen in vitro by the SABC Immunological Techniques and Gold-labeled Colloid Electron Microscopy Method / 中国病毒学
Virologica Sinica
;
(4): 88-91, 2001.
Artículo
en Chino
| WPRIM
| ID: wpr-635223
ABSTRACT
To study the expression of HCV non-structure 5 antigen in vitro, a human HepG2 cell line was incubated with a HCV RNA positive serum. The S ABC i mmunological techniques and gold-labeled colloid electron microscopy method wer e employed to examine for the viral proteins in those cells. The HCV non-struct ure 5 antigen was first detected in the HepG2 cells at 72 hours post incubation. The antigen was continuously observed in the cytoplasm or on the membrane as we ll on the cell wall of the HepG2 cells even after 1, 2, 3 and 4 weeks post incub ation. The observation of HCV non-structure 5 antigen continuously expressed in the HepG2 cells strongly indicates that the cells may have been infected by HCV virus and the virus may have replicated in the cells. Therefore, the HepG2 cell line may be served as a potential host for establishment of HCV infection and p ropagation in vitro.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Virologica Sinica
Año:
2001
Tipo del documento:
Artículo
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