Molecular Cloning and Sequencing of HCV E2 Gene / 中国病毒学
Virologica Sinica
;
(4): 40-44, 2001.
Artículo
en Chino
| WPRIM
| ID: wpr-635231
ABSTRACT
HCV RNA positive serum was first selected by RT-PCR test kit from several anti-HCV positive sera obtained from Xi'an.HCV RNA extrac ted from the elected sera was converted to cDNA by reverse transcription with ra ndom primer.Half-nested PCR was performed.The amplified product was 852 bp.The purified PCR product was digested by restriction endonucleases and then ligated to epressio vector pET-22b\++.Its nucleotide sequence was determined by dideoxy chain termination method.A comparison of the sequence with several isolates rep orted previously showed that the sequence belonged to HCV type Ⅱ.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Virologica Sinica
Año:
2001
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS