Regulatory Effect of Nitric Oxide on Hydrogen Sulfide/ Cystathionine-?-Lyase of Vascular Smooth Muscle Cells in Spontaneously Hypertensive Rats / 实用儿科临床杂志
Journal of Applied Clinical Pediatrics
; (24)1993.
Article
en Zh
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| ID: wpr-638691
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WPRO
ABSTRACT
Objective To investigate the effect of nitric oxide on hydrogen sulfide/ cystathionine-?-lyase(CSE) system in the vascular smooth muscle cells of spontaneously hypertensive rats(SHR).Methods The SHR aortic smooth muscle cells(SMCs) were divided into 2 groups: sodium nitroprusside(SNP) group and control group.The content of hydrogen sulfide in the culture media was measured by sulfide electrode method,and the CSE mRNA level was assayed by competitive reversed transcription-polymerase chain reaction((RT-PCR)).Results Compared with control group,the content of hydrogen sulfide in the culture media of SNP group was significantly higher [(16.16?3.71) ?mol/L vs(22.71?4.84) ?mol/L],and the CSE mRNA level in SMCs in SNP group was lower than that of control group.Conclusion Nitric oxide exerted complicated effect on the hydrogen sulfide/CSE system in the SHR smooth muscle cells.J Appl Clin Pediatr,2006,21(3):140-141
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Zh
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Journal of Applied Clinical Pediatrics
Año:
1993
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Article