Polymerase Chain Reaction-Sequence Special Primers in Detecting Gene Polymorphism of Pediatrics Diseases / 实用儿科临床杂志
Journal of Applied Clinical Pediatrics
; (24)2004.
Article
en Zh
| WPRIM
| ID: wpr-638776
Biblioteca responsable:
WPRO
ABSTRACT
Objective To establish an approach of polymerase chain reaction-sequence special primers(PCR-SSP) to perform polymorphism analysis for multiple genes simultaneously,in order to be used in clinical analysis.Methods The optimized PCR-SSP approach was used to analyze the polymorphism of the following genes:mutation of-308A/G and-238G/A in tumor necrosis factor-?((TNF-?)),-174G/C in interleukin-6(IL-6).Results Polymorphism analysis of multiple genes and many clinical samples could be simultaneously performed with one PCR program,showing clear genotype,quick and accurate genotype.Conclusion The optimized(PCR-SSP) is suitable for polymorphism analysis of the large-sample polygenic mononcleeotide point mutation,and it can be widely applied in clinical test for low cost,quickness and accuracy.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Journal of Applied Clinical Pediatrics
Año:
2004
Tipo del documento:
Article