Application of Different Experimental Methods for Determining Neural Stem Cells in Hippocampus from Human Fetal Brain / 实用儿科临床杂志
Journal of Applied Clinical Pediatrics
; (24)2006.
Article
en Zh
| WPRIM
| ID: wpr-638828
Biblioteca responsable:
WPRO
ABSTRACT
Objective To explore a precise and dependable method for determining neural stem cells(NSCs)in hippocampus from human fetal brain.Methods Ten cases of embryo at gestational age 32 weeks and induction of labor with water bag were collected,affused and sliced including frost slice and paraffin slice for determining alteration of tissue and NSCs in hippocampus from human fetal brain with HE stain and immunohistochemical staining under light microscope.Results Nestin proteins located in NSCs and the number of NSCs were less in paraffin slice of tissue than those in frost slice.Integrality of structure of tissues and cells in paraffin slice excelled in frost slice.Conclusions There is a precise and dependable method including induction of labor with water bag and allusion and frost slice,which is necessary for wide useness in study of NSCs in human fetal brain.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Journal of Applied Clinical Pediatrics
Año:
2006
Tipo del documento:
Article