Expression of Transcription Factor and Cytokine of Th1/Th2 in Children with Asthma in Remission Stage / 实用儿科临床杂志

ABSTRACT

Objective To evaluate the balance of Th1/Th2 cells in the period of remission stage of asthma from the aspects of genes and protein.Methods Peripheral blood from 24 children with asthma in remission stage(asthma group) was obtained.Cytokines interfe-(ron-?)(IFN-?) and interleukin-4(IL-4) were measured by enzyme linked immunosorbent assay(ELISA).The expression of T-bet mRNA and GATA-3 mRNA in peripheral blood mononuclear Cells(PBMCs) were measured by real-time quantitative polymerase chain reaction(PCR).Another 12 healthy children were chosen as control group.Results The plasma level of IL-4 in asthma group was significantly higher than that in control group[(37.14?12.77) ng/L vs(27.08?6.12) ng/L,t=2.467 P