Protein Kinase C Regulating Activation on Nuclear Factor-?B in T Lymphocyte in Children with Acute Idiopathic Thrombocytopenic Purpura / 实用儿科临床杂志
Journal of Applied Clinical Pediatrics
; (24)2006.
Article
en Zh
| WPRIM
| ID: wpr-639025
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WPRO
ABSTRACT
Objective To explore the role of protein kinase C(PKC) to regulate the activation of nuclear factor-?B(NF-?B)in T lymphocyte in children with acute idiopathic thrombocytopenic purpura(ITP).Methods Sterility peripheral blood was collected from acute ITP children(n=30)and healthy children(n=30).T lymphocytes were isolated and purified,and divided into 3 groups:control group,PMA group stimulated with PMA,PMA plus H-7 group stimulated with PMA and H-7.The expression of NF-?B and inhibitor protein-?B(I-?B)was detected by immunohistochemical staining and Western blot,respectively.Results The percentage of cells with active NF-?B was significantly higher and the expression level of I-?B was significantly lower in acute ITP PMA group than that in acute ITP control group and normal PMA group,respectively(all P
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WPRIM
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Zh
Revista:
Journal of Applied Clinical Pediatrics
Año:
2006
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Article