Changes of Expression and Activity of Heme Oxygenase-1 in Lung Tissue of Hyperoxia-Induced Preterm Rats / 实用儿科临床杂志
Journal of Applied Clinical Pediatrics
;
(24)1992.
Artículo
en Chino
| WPRIM
| ID: wpr-639905
ABSTRACT
Objective To observe the changes of expression and activity of heme oxygenase-1(HO-1) in lung tissue of preterm rats exposed to hyperoxia,and explore the role of HO-1 in hyperexia-induced lung injury of preterm rats.Methods Three-day-old preterm rats of standard SD were randomly assigned to hyperoxia group and air group.At the third and 7th day of exposure,the expressions of HO-1 mRNA were detected by means of reverse transcription polymerase chain reaction and the cellular distribution and expressions of HO-1 protein in the lung were measured by immunohistochemical techniques,respectivesly,and the active of HO-1 was determined also.Results On the third day,in air group,the expressions of HO-1 mRNA(0.17 ?0.08),HO-1(7.23?4.63)were mildly expressed and the activity of HO-1 was(4.32?1.57) nmol/(mg?h);compared with those of air group,the expression of HO-1 mRNA in hyperoxia group(0.72?0.33) was significantly increased(Pa
Texto completo:
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Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Journal of Applied Clinical Pediatrics
Año:
1992
Tipo del documento:
Artículo
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