Experimental Study on Early Diagnosis and Treatnent of Gentamicin Ototoxicity / 上海第二医科大学学报

ABSTRACT

Objective Distortion product otoaconstic emission (DPOAE) was compared withauditory brain－stem response (ABR) in the early diagnosis of gentamicin (GM) ototoxicity. Whetherimpaired hearing could be improved with the glutathione treatment after its withdrawl of injection.Methods40 guinea pigs with normal hearing were randomly divided into 4 groups, 10 in DPOAEgroup, 12 in ABR I group (the usage of GM was stopped when abnormal shift presented in ABR), 12 inABR Ⅱ (group glutathione was injected for 5 after the usage of GM had been stopped), 6 in controlgroup. The guinea pigs in experimental group were injected with GM 100mg/kg per day, while those inthe control group were injected with saline. The DPOAE and ABR were monitored to observe the ampli-tude and the threshold of wave IV. The morphological changes of hair cells were also observed after injec-tion of GM had been stopped for 2 weeks. ResultsIt was averaged at 7d after the injection of GMwhen the changes presented in DPOAE group, while 10d in ABR group. The threshold shift increasedafter the injection had been stopped for 2 weeks in ABR Ⅰ group ( P <0.01) ,while there was no obvi-ous shift in ABR Ⅱ group. The morphological and functional changes of the hair cells were almost thesame. Conclusion GM ototoxicity could be more sensitively observed by DPOAE than ABR. Glu-tathione could prevent further ototoxic effect of GM to the cochlear.