Your browser doesn't support javascript.
loading
The effects of Bio-Oss(R) as a scaffolds during sinus bone graft using mesenchymal stem cells in rabbit
Journal of the Korean Association of Oral and Maxillofacial Surgeons ; : 405-418, 2007.
Artículo en Coreano | WPRIM | ID: wpr-64109
ABSTRACT
Mesenchymal stem cells (MSCs) have been though to be multipotent cells that can replicate that have the potential to differentiate into lineages of mesenchymal tissue including the bone, cartilage, fat, tendon, muscle, and marrow stroma. Especially, scaffolds to support cell-based tissue engineering are critical determinants of clinical efforts to regenerate and repair the body. Selection of a matrix carrier imvolves consideration of the matrix's role as a scaffold for physical support and host tissue integration as well as its ability to support of synergize the osteoinductive program of the implanted mesenchymal stem cell. The aim of this study is to evaluate the effect of autobone and Bio-Oss(R) to adherent mesenchymal stem cells as scaffolds on sinus augmentation with fibrin glue mixture in a rabbit model. 16 New Zealand White rabbits were divided randomly into 4 groups based on their time of sacrifice (1, 2, 4 and 8 weeks). First, mesenchymal stem cells were isolated from iliac crest marrow of rabbits and expanded in vitro. Cell culture was performed in accordance with the technique described by Tsutsumi et al. In the present study, the animals were sacrificed at 1, 2, 4 and 8 weeks after transplantation, and the bone formation ability of each sides was evaluated clinically, radiologically, histologically and histomorphologically. According to the histological observations, autobone scaffolds group showed integrated graft bone with host bone from sinus wall. At 2 and 4 weeks, it showed active newly formed bone and neovascularization. At 8 weeks, lamellae bone was observed in sinus graft material area. Radiologically, autobone with stem cell showed more radiopaque than Bio-Oss(R) scaffolds group. there were significant differences in bone volume between 4 and 8 weeks (p<0.05).
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Osteogénesis / Células Madre / Tendones / Médula Ósea / Cartílago / Adhesivo de Tejido de Fibrina / Técnicas de Cultivo de Célula / Trasplantes / Ingeniería de Tejidos / Células Madre Mesenquimatosas Límite: Animales Idioma: Coreano Revista: Journal of the Korean Association of Oral and Maxillofacial Surgeons Año: 2007 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Osteogénesis / Células Madre / Tendones / Médula Ósea / Cartílago / Adhesivo de Tejido de Fibrina / Técnicas de Cultivo de Célula / Trasplantes / Ingeniería de Tejidos / Células Madre Mesenquimatosas Límite: Animales Idioma: Coreano Revista: Journal of the Korean Association of Oral and Maxillofacial Surgeons Año: 2007 Tipo del documento: Artículo