Gene clone and activity assay of apoptin / 军事医学科学院院刊
Bulletin of The Academy of Military Medical Sciences
; (6): 85-88, 2001.
Article
en Zh
| WPRIM
| ID: wpr-642346
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WPRO
ABSTRACT
Objective:To obtain apoptin gene and to induce tumor cell (HeLa) apoptosis. Methods:Apoptin gene was amplified by PCR from CAV TK5803 genomic DNA,and was then cloned into pCDNA3.1/His/Topo vector. After confirming by DNA sequencing, apoptin gene was subcloned into pCIneo vector.Recombinant plasmid pCDNA3.1/His/Topo-apoptin and pCIneo-apoptin were transformed into HeLa cells by Lipofectamine reagent. Three days after transfection,the HeLa cell was stained by Honchest 33258 and apoptosis was analysed by fluorescence microscope. Results and Conclusions: The full-length apoptin gene was cloned by PCR and inserted into pCDNA3.1/His/Topo vector. Sequence analysis demonstrated that it was same as published apoptin gene sequence. Three days after transfection, HeLa cell turned into apoptosis.
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WPRIM
Idioma:
Zh
Revista:
Bulletin of The Academy of Military Medical Sciences
Año:
2001
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Article