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Improvement of rat pulmonary microvascular endothelial cell primary culture methods / 军事医学科学院院刊
Article en Zh | WPRIM | ID: wpr-642524
Biblioteca responsable: WPRO
ABSTRACT
Objective:To improve the primary culture method of rat pulmonary microvascular endothelial cells(PMVECs) and obtain purified PMVECs.Methods:The modified tissue block pasted culture method was used to isolate and culture Wistar rat PMVECs. The morphous of cultured cells were observed by microscopy. The cultured cells were identified by detecting factor Ⅷ related antigen and binding isolectin B4. Results and Conclusion:The morphous of cultured primary PMVECs in vitro showed short fusiform shape or polygon, and the monolayer of cultured cells displayed the shape of pavingstone. But the morphous changed followed the transfer of culture and the change of culture condition. The cultured cells had characterization of binding isolectin B4 and negative immunocytochemical staining for factor Ⅷ related antigen. The cultured PMVECs have good growth state and purity,and can be subcultringed stably.The observation of cell morphous integrating with immunocytochemical staining is a reasonable identification method for PMVECs.
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Texto completo: 1 Índice: WPRIM Tipo de estudio: Prognostic_studies Idioma: Zh Revista: Bulletin of The Academy of Military Medical Sciences Año: 2009 Tipo del documento: Article
Texto completo: 1 Índice: WPRIM Tipo de estudio: Prognostic_studies Idioma: Zh Revista: Bulletin of The Academy of Military Medical Sciences Año: 2009 Tipo del documento: Article