Axon Formation Follows Dendritic Differentiation in the Cultured Ventral Spinal Cord Neurons of Rat / 대한해부학회지
Korean Journal of Anatomy
;
: 181-189, 1998.
Artículo
en Coreano
| WPRIM
| ID: wpr-644105
ABSTRACT
To understand the early cellular differentiation of neurons, we studied the differentiation of ventral spinal cord (VSC) neurons in culture. Immunofluorescence techniques with myelin associated protein 2 (MAP2) and phosphorylated neurofilament heavy chain were used with phase contrast microscopy. VSC neurons were best grown and differentiated on the coverslips coated with polyethylenimine or poly-L-Lysine. During 3 days of culture, VSC neurons changed from a round cell with no neurites to multipolar neurons with an axon and dendrites. The differentiating VSC neurons could be classified into 4 types based on the shape and length of processes. The process with axonal character, that is MAP2 negative and phosphorylated neurofilament positive, was first identified at the tip of dendritic process when one or more processes grew out. Our results suggest that the formation of an axon in VSC neurons may follow the formation of dendrites.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Polietileneimina
/
Médula Espinal
/
Axones
/
Microscopía Electrónica de Rastreo
/
Técnica del Anticuerpo Fluorescente
/
Microscopía de Contraste de Fase
/
Neuritas
/
Técnicas de Cultivo de Célula
/
Dendritas
/
Vaina de Mielina
Límite:
Animales
Idioma:
Coreano
Revista:
Korean Journal of Anatomy
Año:
1998
Tipo del documento:
Artículo
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