Characterization of Clostridium difficile Strains Isolated from Patients with C. difficile-associated Disease in Korea
Osong Public Health and Research Perspectives
;
(6): 325-331, 2017.
Artículo
en Inglés
| WPRIM
| ID: wpr-644319
ABSTRACT
OBJECTIVES:
Studies on Clostridium difficile are rare in Korea. We investigated the epidemiological characteristics of C. difficile isolates from patients with C. difficile-associated disease (CDAD) in Korea.METHODS:
Multiplex polymerase chain reaction was performed to detect the presence of tcdA and tcdB toxin genes. Antimicrobial susceptibility test was carried out by the disk-dilution method. C. difficile strains were subtyped by automated repetitive-element palindromic PCR (rep-PCR).RESULTS:
Among patients with CDAD, 73 (25.8%), 32 (11.3%), 32 (11.3%), and 26 (9.2%) suffered from pneumonia, cancer or neoplasm, diabetes, and colitis, respectively. Of all stool samples, 43 samples (15.2%) were positive for C. difficile strains. We observed two expression patterns of toxin genes tcdA+/tcdB+ (86% isolates) and tcdA−/tcdB+ (14% isolates), with all isolates expressing tcdB. Furthermore, some isolates were resistant to clindamycin (65%), ampicillin (56%), and cefazolin (40%), but all were susceptible to vancomycin and metronidazole. The tested samples were classified into diverse clusters using automated rep-PCR.CONCLUSION:
Our findings revealed the characteristics and antibiotic resistance of C. difficile isolates from patients in Korea. The epidemiological data may provide valuable insight into development of treatment strategies for C. difficile infections in Korea.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Neumonía
/
Farmacorresistencia Microbiana
/
Clindamicina
/
Vancomicina
/
Cefazolina
/
Reacción en Cadena de la Polimerasa
/
Clostridioides difficile
/
Clostridium
/
Colitis
/
Reacción en Cadena de la Polimerasa Multiplex
Límite:
Humanos
País/Región como asunto:
Asia
Idioma:
Inglés
Revista:
Osong Public Health and Research Perspectives
Año:
2017
Tipo del documento:
Artículo
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