Effect of Polyinosinic-Polycytidylic Acid on MUC5B Expression in Human Airway Epithelial Cells / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
; : 615-621, 2015.
Article
en Ko
| WPRIM
| ID: wpr-645459
Biblioteca responsable:
WPRO
ABSTRACT
BACKGROUND AND OBJECTIVES: Polyinosinic-polycytidylic acid (Poly I:C) is structurally similar to double-stranded RNA, and is known to induce various inflammatory mediators and to cause inflammatory reactions in airway epithelial cells. However, the effect of Poly I:C on secretion of mucins in human airway epithelial cells has been very rarely reported. In this study, the effect and brief signaling pathway of Poly I:C on the expression of mucin genes were investigated in human airway epithelial cells. MATERIALS AND METHOD: In mucin-producing human NCI-H292 airway epithelial cells and the primary cultures of normal human nasal epithelial cells, the effect and signaling pathway of Poly I:C on expression of mucin genes were investigated using reverse transcriptase-polymerase chain reaction, real-time PCR, enzyme immunoassay, and immunoblot analysis with specific inhibitors and small interfering RNA (siRNA) for mitogen-activated protein kinase (MAPK). RESULTS: Poly I:C induced the MUC5B expression, and activated the phosphorylation of ERK1/2 and p38 MAPK. U0126 (ERK1/2 MAPK inhibitor) and SB203580 (p38 MAPK inhibitor) inhibited the Poly I:C-induced MUC5B expression. In addition, the knockdown of ERK2 and p38 MAPK by siRNA significantly blocked the Poly I:C-induced MUC5B mRNA expression. CONCLUSION: Poly I:C induces the MUC5B expression via ERK2 and p38 MAPK signaling pathways in human airway epithelial cells. Therefore, Poly I:C may play a role in the regulation of mucus hypersecretion through MAPK signaling pathways in the human airway epithelial cells.
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Texto completo:
1
Índice:
WPRIM
Asunto principal:
Fosforilación
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Proteínas Quinasas
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ARN Bicatenario
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ARN Mensajero
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Técnicas para Inmunoenzimas
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Poli I-C
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ARN Interferente Pequeño
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Proteínas Quinasas p38 Activadas por Mitógenos
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Células Epiteliales
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Reacción en Cadena en Tiempo Real de la Polimerasa
Límite:
Humans
Idioma:
Ko
Revista:
Korean Journal of Otolaryngology - Head and Neck Surgery
Año:
2015
Tipo del documento:
Article