Expression of Heat Shock Protein 70 in Influenza A Virus Induced Acute Maxillary Sinusitis of Rabbits / 대한이비인후과학회지

ABSTRACT

BACKGROUND: Heat shock proteins(HSPs) are released from the cells by temperature elevation or other stresses, including cytokines, hypoxia, inflammation or reactive oxygen species. These proteins likely play a role in cellular repair and survival mechanisms. OBJECTIVES: In order to elucidate the relationship between the HSP 70 and viral induced maxillary sinusitis. MATERIALS AND METHODS: The degree of HSP expression was evaluated in New Zealand white rabbits with maxillary sinusitis induced by inoculation of Influenza A virus(KOREA /1/ 80/H3N2) into the mucosa. The animals were serially sacrificed 1, 5, 7, 14, 21, 28th day after inoculation. The localization of the induced form of HSP 70 in the normal & infected maxillary mucosa were studied with immunohistochemical staining. RESULTS: The normal sinus mucosa did not show any staining for the HSP 70. As contrasted with the normal group, the mucosa of the first day after viral inoculation showed very light staining in the epithelial layer. The degree of immunoreactive staining was gradually increased up to the seventh day. Epithelial layer of the mucosa, cilia and submucosa were heavily stained at seventh day after inoculation, and then the degree was reduced at 14th day. The staining of the mucosa was completely disappeared at 28th day after viral inoculation. CONCLUSION: These results suggest that the HSP 70 was produced from acute stage of the infected mucosa by the Influenza A virus. Presumably, it is closely related to the inflammatory reaction in the mucosa of the maxillary sinus.