Effect of Photostimulation Using Low-Level Laser Therapy on Wound Healing in Induced Mouse Skin Wound / 대한이비인후과학회지

ABSTRACT

BACKGROUND AND OBJECTIVES: This study was conducted to investigate the healing effect of the low-level laser irradiation on wound healing in vivo using DPSS laser (532 nm) and Diode laser (660 nm). MATERIALS AND METHOD: Each mouse received dorsal, full-thickness round incision (=2 cm) and daily laser irradiation (4 J/cm2) was done before sacrifice. On sacrifice at 3, 7, 10 days, the wound was excised, then wound closure and histologic stages were measured, and standardized. RESULTS: The percentages of wound closure in DPSS laser, Diode laser, control were 33.2+/-2.4, 34.2+/-3.5, 24.0+/-2.7 at day 3, 64.8+/-3.5, 72.2+/-2.8, 42.8+/-5.0 at day 7 and 82.2+/-7.9, 87.2+/-3.7, 71.4+/-4.0 at day 10, respectively, with p<0.05. Histological evaluation showed that laser irradiation enhanced wound epithelialization, cellular content deposition, granulation tissue formation, collagen deposition and neovascularization in the laser-treated wounds as compared to the control group. CONCLUSION: Low-level laser irradiation at 532 nm and 660 nm significantly enhanced cutaneous wound healing effect in the wounded mouse model. Further investigation of the mechanism of low-level laser therapy in primary wound healing is warranted.