Functional Studies of Acyl-CoA Synthetase 4 in the Rat Liver
The Korean Journal of Nutrition
;
: 376-381, 2003.
Artículo
en Coreano
| WPRIM
| ID: wpr-647903
ABSTRACT
Acyl-CoA synthetase 4 (ACS4) is an arachidonate-preferring enzyme abundant in steroidogenic tissues. We examined ACS4 in rat liver, which contains a variety of pathways that use acyl-CoAs, in order to determine subcellular locations. We demonstrate that ACS4 protein was present most abundantly in the mitochondria and to a much lesser extent in the peroxisomes and microsomes. To determined the dietary effects on the level of ACS4 mRNA, northern blotting was carried out using total RNA from the livers of adult male rats fed various diets. Fasting, high fat diet, and fat-free high sucrose diet increased the hepatic level of ACS4 mRNA approximately 2-fold. Furthermore, the levels of ACS4 mRNA were induced by DEHP[Di- (2-ethylhexyl) phthalate]. These data suggest that ACS4 expression in the liver is regulated with a variety of pathways, including beta-oxidation, hormone, and insulin.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Sacarosa
/
ARN
/
ARN Mensajero
/
Western Blotting
/
Northern Blotting
/
Ayuno
/
Ácido Araquidónico
/
Peroxisomas
/
Dieta
/
Dieta Alta en Grasa
Límite:
Animales
/
Humanos
/
Masculino
Idioma:
Coreano
Revista:
The Korean Journal of Nutrition
Año:
2003
Tipo del documento:
Artículo
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