The Control of Tumor Cell Invasiveness in Chondrosarcoma Cell Lines by Modifying Focal Adhesion Kinase Expression / 대한정형외과학회잡지
The Journal of the Korean Orthopaedic Association
; : 552-558, 2002.
Article
en Ko
| WPRIM
| ID: wpr-648185
Biblioteca responsable:
WPRO
ABSTRACT
PURPOSE: We propose that cell attachment and invasion can be regulated by the modulation of FAK expression in chondrosarcoma cell lines. MATERIALS AND METHODS: The C-terminal domain of FAK (FAK-CD) was transfected by recombinant adenovirus infection in chondrosarcoma cell lines, JJ012 and 105KC. The expression of FAK, FAK-CD and tyrosine phosphorylation were checked. Chondrocytes and chondrosarcoma cells were used in cell attachment tests by blocking or not blocking integrin-beta 1 antibodies and synthetic peptides on type II collagen. To evaluate the effect of cell invasiveness, a wound healing assay and a Boyden chamber assay were done after FAKCD transfection. RESULTS: We observed higher FAK expression in the chondrosarcoma cells than in chondrocytes. The level of attachment to type II collagen was significantly inhibited by blocking with the antibody of integrin-beta 1 and synthetic RGD peptides. Also, the adenovirus mediated transfection of FAK-CD resulted in the inhibition of the phosphorylation of FAK and significant inhibition of cell attachment in only JJ012, without changing FAK expression. Moreover, migration after transfection with FAK-CD was reduced by up to 79.9% for JJ012 and 75.5% for 105KC. CONCLUSION: Attachment of chondrosarcoma cells could be mediated through integrin-beta 1. We conclude that modified FAK expression contributes to the suppression of tumor cell attachment and invasion.
Palabras clave
Texto completo:
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Índice:
WPRIM
Asunto principal:
Péptidos
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Fosforilación
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Tirosina
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Cicatrización de Heridas
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Transfección
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Línea Celular
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Adenoviridae
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Condrosarcoma
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Infecciones por Adenoviridae
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Condrocitos
Idioma:
Ko
Revista:
The Journal of the Korean Orthopaedic Association
Año:
2002
Tipo del documento:
Article