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Anti-Oxidative and Anti-Inflammatory Effect of Water Extract from Perillae semen in RBL-2H3 Cells
The Korean Journal of Nutrition ; : 367-373, 2010.
Artículo en Coreano | WPRIM | ID: wpr-648890
ABSTRACT
The present study was to investigate anti-oxidative and anti-inflammatory activity of Perillae semen in RBL-2H3 basophilic leukemia cells. Inhibitory effect of Perillae semen onto free radical generation was determined by measuring DPPH and hydroxyl radical scavenging activities in vitro. Anti-inflammatory actions of Perillae semen extracts (100, 250, 500 microgram/mL) were assessed by testing their effects on the degranulation of mast cells. For this, beta-hexosaminidase released from RBL-2H3 cells was used and proinflammatory cytokines were measured by an ELISA kit. Our results indicated that Perillae semen water extracts effectively inhibited free radical generation. At the concentration of 500 microgram/mL of water extract, the degranulation of RBL-2H3 cells were inhibited by 42.1%. The IgE-antigen complex increased the accumulation of IL-4 and TNF-alpha secretion in RBL-2H3 cells and treatments with 250 and 500 microgram/mL of Perillae semen extracts suppressed the IgE induced secretion of IL-4 and TNF-alpha protein by 20.5, 26.9% and 14.5, 16.5% respectively. We observed that Perillae semen water extract reduced beta-hexosaminidase, IL-4, and TNF-alpha secretion in RBL-2H3 cells. These results provide that Perillae semen may be beneficial in the treatment of allergic inflammatory disease.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Semen / Basófilos / Beta-N-Acetilhexosaminidasas / Inmunoglobulina E / Ensayo de Inmunoadsorción Enzimática / Agua / Leucemia / Citocinas / Interleucina-4 / Factor de Necrosis Tumoral alfa Idioma: Coreano Revista: The Korean Journal of Nutrition Año: 2010 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Semen / Basófilos / Beta-N-Acetilhexosaminidasas / Inmunoglobulina E / Ensayo de Inmunoadsorción Enzimática / Agua / Leucemia / Citocinas / Interleucina-4 / Factor de Necrosis Tumoral alfa Idioma: Coreano Revista: The Korean Journal of Nutrition Año: 2010 Tipo del documento: Artículo