The Effect of reconstituted basement membrane on the expression of the anterior pituitary hormones of the rat / 대한해부학회지
Korean Journal of Anatomy
;
: 365-372, 2000.
Artículo
en Coreano
| WPRIM
| ID: wpr-649729
ABSTRACT
This study was performed to investigate the effect of Matrigel, a reconstituted basement membrane, on the expression of the anterior pituitary hormones in culture. Rat pituitary cells cultured for 6 days on Matrigel showed 3-dimensional, lobular structures with connecting cells while those on plastic showed flat, polygonal cells forming a monolayer. Western blot analysis showed that prolactin (PRL) content in the anterior pituitary cells was higher compared to those cultured on plastic. In comparison, TSH expression was not increased in cultures on Matrigel. The total cell number and the proportion of fibroblasts was decreased. These results suggested that Matrigel is a useful culture substrate for the enhanced expression of PRL but not for TSH. Further studies are needed in order to find a useful culture substrate for TSH cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Hormonas Adenohipofisarias
/
Plásticos
/
Prolactina
/
Membrana Basal
/
Recuento de Células
/
Western Blotting
/
Tirotrofos
/
Fibroblastos
Límite:
Animales
Idioma:
Coreano
Revista:
Korean Journal of Anatomy
Año:
2000
Tipo del documento:
Artículo
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