Analysis of Electrophysiological Change in the Rat LC Nucleus after Salicylic Acid Treatment / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
;
: 148-152, 2006.
Artículo
en Coreano
| WPRIM
| ID: wpr-650939
ABSTRACT
BACKGROUND AND OBJECTIVES:
The precise mechanism of salicylic acid induced tinnitus has not been clearly identified as yet in spite of wide range of studies undertaken. We looked for the electrophysiologic evidence that salicylic acid has effect on the Locus Coeruleus (LC) neurons in vitro. MATERIALS ANDMETHOD:
In LC, we measured the neuronal firing rate and cell membrane property according to the concentration of salicylic acid with extracellular single unit recording and whole cell current clamp recording.RESULTS:
The basal firing activity was increased in 15 of the 20 LC nuclei, which were treated with 0.3 mM salicylic acid. Both 1mM and 2 mM salicylic acid increased the basal firing rate of all except for one LC neuron (n=20). These neurons also showed recovery after washing. However, 5 mM salicylic acid induced cell death after the bursting response in all of the LC neurons (n=10)(Fig. 2). There were no specific changes in the whole cell current-clamp recording of the LC neurons during the period of drug treatment (Fig. 3).CONCLUSION:
The dose dependent response pattern observed in the extracellular single unit recording and the fact that there were no specific changes in the whole-cell current-clamp recording following the salicylic acid treatment suggest that the salicylic acid induced intracellular change in the LC neuron is caused not by the direct ligand-receptor reaction but by the indirect 2nd messenger system.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Acúfeno
/
Locus Coeruleus
/
Membrana Celular
/
Muerte Celular
/
Técnicas de Placa-Clamp
/
Ácido Salicílico
/
Incendios
/
Neuronas
Límite:
Animales
Idioma:
Coreano
Revista:
Korean Journal of Otolaryngology - Head and Neck Surgery
Año:
2006
Tipo del documento:
Artículo
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