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The Role of Calcium Affecting Signal Pathway Related to Nitric Oxide-induced Cytotoxicity in H9c2 Cardiac Myoblast / 대한해부학회지
Korean Journal of Anatomy ; : 587-594, 2000.
Artículo en Coreano | WPRIM | ID: wpr-651161
ABSTRACT
Nitric oxide (NO) elevates intracellular calcium. But the actions of calcium in NO-induced cell death are not well understood. This study was carried out to investigate the signal transduction pathways of calcium and NO-induced cytotoxicity in H9c2 cardiac myoblasts by using NO donor compounds such as sodium nitroprusside (SNP) and S-nitroso-N-acetylpenicillamine (SNAP). Pretreatment of intracellular calcium chelating agent (BAPTA/AM) or L-type calcium channel blockers (nicardipine, nifedipine, diltiazem and veraparmil) or T-type calcium channel blocker (flunarizine) blocked SNP-induced cytotoxicity respectively only in a three hours. However, thapsigargin (TG), which inhibits endoplasmic reticulum dependent Ca(2+)-ATPase and thereby increases cytosolic Ca(2+), augmented SNP-induced cytotoxicity. The protective effect of BAPTA/AM was inhibited by treatment of protein synthesis inhibitor, cyclohexamide. In addition, pyrrolidine dithiocarbamate (PDTC), NF-kB inhibitor, attenuates the protective effect of BAPTA/AM against SNP-induced cytotoxicity. It is indicated that the protective effect of BAPTA/AM against NO-induced cytotoxicity might be due to the expression of protein related to activation of NFkB. From these results, it is concluded that SNP-induced cytotoxicity is mediated by calcium in a 3 hours via down regulation of protein expression rleated to activation of NFkB.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Donantes de Tejidos / Nitroprusiato / Diltiazem / Nifedipino / Transducción de Señal / Regulación hacia Abajo / Calcio / FN-kappa B / Muerte Celular / Tapsigargina Límite: Humanos Idioma: Coreano Revista: Korean Journal of Anatomy Año: 2000 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Donantes de Tejidos / Nitroprusiato / Diltiazem / Nifedipino / Transducción de Señal / Regulación hacia Abajo / Calcio / FN-kappa B / Muerte Celular / Tapsigargina Límite: Humanos Idioma: Coreano Revista: Korean Journal of Anatomy Año: 2000 Tipo del documento: Artículo