Expresion of Collagenase-3 (Matrix Metalloproteinase-13) in human middle ear cholesteatoma and granulation / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
; : 572-575, 2001.
Article
en Ko
| WPRIM
| ID: wpr-651386
Biblioteca responsable:
WPRO
ABSTRACT
INTRODUCTION: Middle ear cholesteatoma is notorious for its capacity to absorb surrounding bone, but the exact mechanism of bone destruction is not fully understood until now. Recently, a group of matrix metalloproteinases (MMPs) were found to dissolve extracellular matrices. Among them, collagenase-3 (MMP-13), a newly found collagenase, is thought to be a strong enzyme to dissolve bone and cartilage. So we tried to find out whether Collagenase-3 is found in cholesteatoma and plays some role in bone destruction by cholesteatoma. MATERIALS AND METHOD: We performed immunohistochemical stains on 5 cholesteatomas, 5 middle ear granulations, 5 normal middle ear mucosa and 5 deep meatal skin specimens with anticollagenase-3 antibody and analysed the staining patterns. RESULTS: All the cholesteatomas showed strong immunoreactivity with collagenase-3. Two out of 5 granulations showed somewhat weaker and more disseminated patterns of immunoreactivity, but the rest 3 granulations showed no immunoreactivity. Normal middle ear mucosa and deep meatal skin specimens showed no immunoreactivity at all. CONCLUSIONS: Collagenase-3 may play an active role in the process of bone destruction by cholesteatoma; however, a further study using zymography or blotting method is needed to make this clear.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Piel
/
Cartílago
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Colesteatoma
/
Colagenasas
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Colesteatoma del Oído Medio
/
Metaloproteinasas de la Matriz
/
Colorantes
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Oído Medio
/
Matriz Extracelular
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Metaloproteinasa 13 de la Matriz
Límite:
Humans
Idioma:
Ko
Revista:
Korean Journal of Otolaryngology - Head and Neck Surgery
Año:
2001
Tipo del documento:
Article